IUBio

Amino acid analysis problems

ummanley at cc.UManitoba.CA ummanley at cc.UManitoba.CA
Thu Mar 4 11:28:14 EST 1999


Sorry.....

I express in the E.coli strain BL21(DE3)pLysS.  Actually I use a 4M urea/
SDS mixture for solubilization (separately the urea and SDS are
inefficient at solubilizing my protein, the best detergent is
dodecylmaltoside.....but its very expensive). The protein is a natural
E.coli membrane protein, and there is no evidence of inclusion bodies
(even EM doesn't show anything). 
 
Do you suggest removing the SDS instead of the urea for AAA?

Thanks, 

Darren

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{                                                                }
{ Darren Manley                         Department of Chemistry  }
{ phone (204) 474-6697                  University of Manitoba   }
{ fax (204) 474-7608                    Winnipeg, MB., R3T-2N2   }
{ email ummanley at cc.umanitoba.ca        Canada                   }
{                                                                }
{                                                                } 
{        Quantum Mechanics: The dreams stuff is made of.         }
{                                                                }
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