IUBio

Amino acid analysis problems

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Thu Mar 4 10:26:36 EST 1999


Darren, you don't write in what host you express your protein. If 
it's E. coli, you could try 2 to 6M urea or guanidinium salts for 
solubilizing your inclusion bodies. 

Wolfgang
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Wolfgang Schechinger   
Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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