How to handle PAS?

Rich Dudley rdudley+ at pitt.edu
Thu Mar 4 09:44:11 EST 1999


Wiebke Schmidt wrote:

> I want to compare the activities of several mutants of an enzyme. In the
> assay immunoprecitates of the enzyme are used. I have problems in using
> equal amounts of immunoprecipitate in each assay because of the
> consistence of the protein A-Sepharose (PAS). Does anyone have a
> suggestion how to pipette  equal amounts of PAS?
> Thanks in advance,
> Wiebke

I cut the end off of a 200 ul tip and use that.  Works fairly well.

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
---> search BIONET archives at http://www.bio.net <---





More information about the Methods mailing list