protocol for total RNA purification from placental tissue

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Thu Mar 4 17:38:36 EST 1999

rashie athukorala wrote:

> can anyone suggest a protocol or reasonably priced kit that might work.  I
> plan on doing RT-PCR on the RNA.
> thanks.

Fire up Medline and hunt down papers by people at the Toxicology
Department in Oulu (Finland) - eg. Hannu Raunio, Olavi Pelkonen,
Markku Pasanen etc.  These guys have been working on drug
metabolising enzymes in placental samples for years.  Last time
I heard Hannu talk he had some really nice RT-PCR data from
placentae so their methodology must be good.

Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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