precipitating proteins w/ ethanol

Katherine Walstrom kwalstro at virtu.sar.usf.edu
Thu Mar 4 18:21:27 EST 1999


I have some dilute protein samples that I need to concentrate and run on
an SDS-PAGE gel.  I can't find a detailed protocol for precipitating
proteins, although what I've read suggests that ethanol works well.  I
want to precipitate all the proteins, not just selectively precipitate
some of them.  Does anyone have a general protocol for this?

Thanks much.

Katherine M. Walstrom, PhD
Division of Natural Sciences
New College of USF
5700 N. Tamiami Trail
Sarasota, FL  34243
kwalstro at sar.usf.edu





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