IUBio

Best his-tag and myc-tag antibodies?

Ian Mc i.mcfarlane at icrf.icnet.uk
Fri Mar 5 05:37:17 EST 1999


> 
> 
> Whilst I haven't used his-tag or myc-tag antibodies, I have recently been
> trying Qiagen's Ni-NTA-AP conjugate and it works pretty well.  You need to
> be quite stringent with the protocol otherwise there can be significant
> background, but on the whole the results are nice.  Main advantage is it's
> around half the price of the anti-His antibodies.
> 
> Neil Saunders
> 

How stringent is quite stringent? Could you post your protocol? I think it
would be of great help to people considering using this conjugate.

Thanks,

Ian Mc



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