>>> Whilst I haven't used his-tag or myc-tag antibodies, I have recently been
> trying Qiagen's Ni-NTA-AP conjugate and it works pretty well. You need to
> be quite stringent with the protocol otherwise there can be significant
> background, but on the whole the results are nice. Main advantage is it's
> around half the price of the anti-His antibodies.
>> Neil Saunders
How stringent is quite stringent? Could you post your protocol? I think it
would be of great help to people considering using this conjugate.