Best his-tag and myc-tag antibodies?

Dr Dave Parcej parcej at biophys.mpg.de
Fri Mar 5 13:01:40 EST 1999


I like to use the Sigma anti-His1 antibody. Works at 1 in 50 000 for us on
blots! As for anti-cmyc we have used the Invitrogen antibody with success
(again on Westerns).
Dave 

In article <36DBED97.B34DE691 at pitt.edu>, rdudley+ at pitt.edu wrote:

> "D. Pearton" wrote:
> 
> > Hi all,
> >
> > I'm soliciting opinions as to the best source of his-tag and myc-tag
> > antibodies.  I've had problems detecting my his-tag fusion protein with
> > the invitrogen antibodies and I was wondering which companies you've had
> > the most success with.
> >
> > Thanks in advance,
> > Yak
> 
> Dave--
> 
> I like Qiagen's Hexa-his antibody.  They also have a penta-his, which in my
> experience gives a little better signal, but higher background (meaning in
> this case spurrious bands).  The hexa-his had a lower signal, but was more
> specific.
> 
> Good luck!
> 
> rich
> 
> --- --- --- -- -- -- --- --- ---
> Richard J. Dudley (rdudley+ at pitt.edu)
> Research Specialist V
> Dept. of Cell Biology and Physiology
> University of Pittsburgh
> http://www.cbp.pitt.edu
> ---> search BIONET archives at http://www.bio.net <---



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