DNA isolation from agarose gels
fitzsp at cais.net
Fri Mar 5 18:37:03 EST 1999
What is the simplest and most efficient method for recovering restriction
fragments from agarose gels. I am trying to recover PCR product whose ends
have been digested with restriction enzymes from HMP agarose. The gels have
loads of product, but recovery of approximately 5% seems to be the norm.
I've tried Wizard PCR preps, Quiex II, and electroelution, all with very
dissappointing yields. Any suggestions of simple, high-yield protocols?
Thanks in advance.
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