DNA isolation from agarose gels

Sean Fitzsimmons fitzsp at cais.net
Fri Mar 5 18:37:03 EST 1999


What is the simplest and most efficient method for recovering restriction
fragments from agarose gels.  I am trying to recover PCR product whose ends
have been digested with restriction enzymes from HMP agarose.  The gels have
loads of product, but recovery of approximately 5% seems to be the norm.
I've tried Wizard PCR preps, Quiex II, and electroelution, all with very
dissappointing yields.  Any suggestions of simple, high-yield protocols?

Thanks in advance.

Sean Fitzsimmons





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