In article <KTZD2.351$6D3.7799136 at newsie.cais.net> "Sean Fitzsimmons" <fitzsp at cais.net> writes:
>What is the simplest and most efficient method for recovering restriction
>fragments from agarose gels. I am trying to recover PCR product whose ends
>have been digested with restriction enzymes from HMP agarose. The gels have
>loads of product, but recovery of approximately 5% seems to be the norm.
>I've tried Wizard PCR preps, Quiex II, and electroelution, all with very
>dissappointing yields. Any suggestions of simple, high-yield protocols?
>>Thanks in advance.
I've had good results with the Qiagen QiaQuick gel extraction kit.