DNA isolation from agarose gels

Annette C. Hollmann ah690549 at bcm.tmc.edu
Sat Mar 6 05:45:51 EST 1999


In article <KTZD2.351$6D3.7799136 at newsie.cais.net> "Sean Fitzsimmons" <fitzsp at cais.net> writes:
>What is the simplest and most efficient method for recovering restriction
>fragments from agarose gels.  I am trying to recover PCR product whose ends
>have been digested with restriction enzymes from HMP agarose.  The gels have
>loads of product, but recovery of approximately 5% seems to be the norm.
>I've tried Wizard PCR preps, Quiex II, and electroelution, all with very
>dissappointing yields.  Any suggestions of simple, high-yield protocols?
>
>Thanks in advance.
>
>Sean Fitzsimmons
>

I've had good results with the Qiagen QiaQuick gel extraction kit.

Annette





































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