Alkaline downward trnasfering

Gen Yan Yang gyang at MED.UNC.EDU
Mon Mar 8 12:51:13 EST 1999


Thanks a lot for the response to my question! Yes, it's no problem to
neglect the denaturation step after depurinization if using 0.4 N NaOH
as transfering buffer,  I just developed my film and got stronger
hybridyzation singal with the band up to 25 kb. 

Yang
the University of North Carolina at Chapel Hill



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