IUBio

Disulfide reduction with TCEP

Kresten Lindorff Larsen kresten at my-dejanews.com
Mon Mar 8 11:36:41 EST 1999


Does anybody have experience with using tris-(2-carboxyethyl) phosphine (TCEP)
or other phosphines for reduction of disulfide bonds in proteins.

Is the reagent easy to handle?

What protocol do you use:
1. n(TCEP):n(protein -SS-)
2. temperature
3. time
4. pH
more?

Where can the reagent be bought

thank you
Kresten


The address kresten at my-dejanews.com is for spambots only. Please mail me
at LysLeuLeu at crc.dk , transforming the pre at -part into my initials.
Kresten Lindorff Larsen, Dept. Yeast Genetics
Carlsberg Laboratory, Denmark

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