DNA isolation from agarose gels

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Mon Mar 8 11:38:48 EST 1999

At 06:37 PM 3/5/99 -0500, Sean Fitzsimmons wrote:
>What is the simplest and most efficient method for recovering restriction
>fragments from agarose gels.  I am trying to recover PCR product whose ends
>have been digested with restriction enzymes from HMP agarose.  The gels have
>loads of product, but recovery of approximately 5% seems to be the norm.
>I've tried Wizard PCR preps, Quiex II, and electroelution, all with very
>dissappointing yields.  Any suggestions of simple, high-yield protocols?
>Thanks in advance.
>Sean Fitzsimmons

Do you know of the simplest method of "freeze squeeze" employing the
microfuge? Gives clean DNA with  recovery approaching 100%. If you don't
have the protocol visit the archives (I had posted it last year) or  write
me back.


Dr. Hiranya Sankar Roychowdhury
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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