precipitating proteins w/ ethanol
pxpst2 at unixs.cis.pitt.edu
Mon Mar 8 10:38:06 EST 1999
In article <i.mcfarlane-0803991440450001 at 184.108.40.206>,
i.mcfarlane at icrf.icnet.uk (Ian Mc) wrote:
> Have you got a protocol or a ref.for this technique?
"Concentration of Dilute protein for gel electrophoresis" Anal. Biochem.
226: 382-383 1995
> I need to recover proteins from an SDS solution and although EtOH and
> acetone will bring them out of solution my usual method of resolubilising
> in 1M Tris pH10.4 isn't working well.
Some proteins do not re hydrate well
> One other thought, do you know if urea precipitation will work in the
> presence of SDS?
Works even in the presense of SDS. Some of the SDS my come thru but for
gels that is not a problem
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