mitochondrial DNA purification

Tony Hodge tph at mrc-lmb.cam.ac.uk
Mon Mar 8 10:31:40 EST 1999


bruno <bruno.delbreil at univ-lille1.fr> wrote:

> Is mitochondrial DNA well separated from nuclear DNA by a single density
> gradient with CsCl (no sucrose gradient before)?

In plants we used to purify the mitochondria by sucrose density first,
DNAse/RNAse, wash then lyse the mitochindria to get mtDNA.

tony

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Tony P Hodge
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