mitochondrial DNA purification

Tony Hodge tph at
Mon Mar 8 10:31:40 EST 1999

bruno <bruno.delbreil at> wrote:

> Is mitochondrial DNA well separated from nuclear DNA by a single density
> gradient with CsCl (no sucrose gradient before)?

In plants we used to purify the mitochondria by sucrose density first,
DNAse/RNAse, wash then lyse the mitochindria to get mtDNA.


Tony P Hodge
tph@*nospam*  (remove *nospam* )

More information about the Methods mailing list