DNA isolation from agarose gels

Gerd gerdn at ibg.uit.no
Mon Mar 8 08:22:58 EST 1999


Sean Fitzsimmons wrote:
> 
> What is the simplest and most efficient method for recovering restriction
> fragments from agarose gels.  I am trying to recover PCR product whose ends
> have been digested with restriction enzymes from HMP agarose. ..
> 
> Sean Fitzsimmons

It would help to know the size of your PCR product.
300 -> 6-7000 bp? Qiagen gel extraction kit beats everything else I have
tried.
Larger fragment? Some freze-squeze protocol might be better.
Gerd



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