mRNA purification

M Modhaddam-matin MBP95MM at sheffield.ac.uk
Mon Mar 8 07:22:43 EST 1999

Dear friends

I am going to make a cDNA library, and it would be grateful if you could
help me with:
1-The best way of large scale extraction/purification of mRNA
2-Amplifying the mRNA to see the bands on the gel.
3-Removal of ribosomal RNA from total RNA, without damage to mRNA.
4-The best way/kit to make cDNA.
5-The best way/kit for construction of library if we want it for 
transfection of mammalian cells.

Many thanks


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