DNA isolation from agarose gels
jladasky at pmgm.Stanford.EDU
Tue Mar 9 01:02:02 EST 1999
In article <KTZD2.351$6D3.7799136 at newsie.cais.net>,
Sean Fitzsimmons <fitzsp at cais.net> wrote:
>What is the simplest and most efficient method for recovering restriction
>fragments from agarose gels. I am trying to recover PCR product whose ends
>have been digested with restriction enzymes from HMP agarose. The gels have
>loads of product, but recovery of approximately 5% seems to be the norm.
>I've tried Wizard PCR preps, Quiex II, and electroelution, all with very
>dissappointing yields. Any suggestions of simple, high-yield protocols?
>Thanks in advance.
I've been using the EluQuik kit from Schleicher and Schuell (no
affiliation). I get yields of approximately 50%. It's the old "glass
milk" protocol, with some improved reagents.
Rainforest laid low.
"Wake up and smell the ozone,"
Says man with chainsaw. - John Ladasky
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