EDTA in growth media

Rafael Maldonado rafamaldo at geocities.com
Mon Mar 8 14:43:19 EST 1999


> In Article <1999Mar3.212220.31589 at ucl.ac.uk>, chen at bsm.bioc.ucl.ac.uk (Chen
> Ho An) wrote:
> >I've seen a couple of recipes of minimal media with EDTA added, but can't
> >work out why it's there.  Does anyone knows the reason?  It occurs to me
> >that perhaps EDTA serves a reservoir of divalent cations which can be
> >released when needed, but surely EDTA has a higher affinity for the cations
> >than enzymes, how else could it inactive enzymes?

I know one case it was for maintaining the Fe ions biologically
available. The Fe must be chelated in a solution of EDTA (free acid)
before adding to the medium.

-Rafa

-- 
Rafael Maldonado
Division of Genetics
University of Alicante, Spain
rmaldonado at ua.es



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