unusual ligation products

[Roger Fachini]

I have seen unusual ligation products during cloning.  Sometimes one of
the sites has a base or two missing, or the gene ligates into the vector
at sites slightly beyond each restritiction site, or the gene somehow goes
in backwards.

I have seen this with different genes and under different conditions, such
as with and without phosphatse treatment of the vector.

Any ideas on the cause(s) of this?

Thanks,

-- 
Roger Fachini
Bacterial Expression
Amgen Research

The opinions expressed are solely the user's and not those of Amgen.