unusual ligation products

Roger Fachini rfachini at amgen.com
Mon Mar 8 17:28:34 EST 1999

I have seen unusual ligation products during cloning.  Sometimes one of
the sites has a base or two missing, or the gene ligates into the vector
at sites slightly beyond each restritiction site, or the gene somehow goes
in backwards.

I have seen this with different genes and under different conditions, such
as with and without phosphatse treatment of the vector.

Any ideas on the cause(s) of this?


Roger Fachini
Bacterial Expression
Amgen Research

The opinions expressed are solely the user's and not those of Amgen.

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