IUBio

Purifying Antibodies using affinity support

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Tue Mar 9 13:16:39 EST 1999


Dima, 

Suppose the periodate protocol is oxidizing cis diols into 
bis aldehydes and reduce the imines formed after coupling with 
cyanoborohydride to amines. And clening leftover CHOs with boranate. 
But this procedure above probably might kill any active enzyme. Is 
there a way to prevent this?
You remember the exact protocol by chance? 

Wolfgang
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Wolfgang Schechinger   
Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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