You are welcome! It's always gratifying to see some advises working!
Malay Kumar Basu
Center for Cellular and Molecular Biology
I N D I A
This system will self-destruct in five minutes.
curiouser at ccmb.ap.ni.in
>Thanks a lot for the response to my question! Yes, it's no problem to
>neglect the denaturation step after depurinization if using 0.4 N NaOH
>as transfering buffer, I just developed my film and got stronger
>hybridyzation singal with the band up to 25 kb.
>the University of North Carolina at Chapel Hill