IUBio

Alkaline downward trnasfering

Malay curiouser at ccmb.ap.nic.in
Tue Mar 9 12:48:23 EST 1999


Dear Yang:

You are welcome! It's always gratifying to see some advises working!

Malay

Malay Kumar Basu
Center for Cellular and Molecular Biology
Hyderabad 500007
I N D I A

Fax: (00-91)40-7171195
Phone: (00-91)40-7172241
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This system will self-destruct in five minutes.
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curiouser at ccmb.ap.ni.in



>Thanks a lot for the response to my question! Yes, it's no problem to
>neglect the denaturation step after depurinization if using 0.4 N NaOH
>as transfering buffer,  I just developed my film and got stronger
>hybridyzation singal with the band up to 25 kb. 
>
>Yang
>the University of North Carolina at Chapel Hill
>




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