IUBio

Ligation, and Transformation iNHIBITORS.

Gerd gerdn at ibg.uit.no
Tue Mar 9 07:25:25 EST 1999


amherrera at CIGB.EDU.CU wrote:
> 
>     Hi all
> 
>     I having a low transformation frequency when transform my
>     ligation?
> 
>     I have evidence about the good efficiency of the competent
>     cells.
> 
>     The vector I used is 10.kb and the band over 3.3kb
> 
>     Thanking Any Suggestion
> 
>     D.Q.V

Read somewhere (Invitrogen cloning kit) that 20 min at 65 C to kill
ligase would help transformation efficiency. Do not have any idéa if it
actually works.

Gerd



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