M9 plate problem

Gerd gerdn at ibg.uit.no
Wed Mar 10 02:23:06 EST 1999

Long wrote:
> Hi, All
> I got a very basic question about M9 plate. Several months ago, I made a
> batch of M9 plates which worked very well. This time, I made another
> batch just following the same receipe and I got a kind of dark brown
> liquid after autoclaving. 

Did you add the carbon source before autoclaving? That could explain the
brownish color - don't know if it will inhibit bacteria.

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