trephine bone marrow biopsy in EDTA

[Wolfgang Schechinger]

Dear Zbigniew, 

What buffer are you using? EDTA complexes Ca and Mg, these ions 
render it uneffective.

You write that your EDTA dissolves at 6%. For pure EDTA (the acid) 
this is not true if you are using neutral range pH buffer (like Tris 
or PBS). I assume that you have the sodium salt (as one obtaines when 
neutralizing EDTA acid with NaOH in order to dissolve it. The pH 
usually then is somewhere between 8 and 10).

If your protocol says "dissolve EDTA by adding NaOH (solid) until 
the solution is clear and you are using sodium EDTA, the pH may be 
the issue, esp. for the calcium ions going into solution. 

Just my 2 ZL/

Wolfgang
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Wolfgang Schechinger   
Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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