allele-specific PCR

Gregor Bucher st2153 at zi.biologie.uni-muenchen.de
Tue Mar 9 12:19:57 EST 1999


Hi!
Does anybody has experience with allele-specific PCR. I´d like to have
some hints how the primers and conditions should be chosen before I start
to find out  these things again. To my knowledge only A-G C-G and A-A
mismaches will give results. 

What else can I do to make the reaction work?

Thanks a lot!

Gregor



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