allele-specific PCR

Gregor Bucher st2153 at
Tue Mar 9 12:19:57 EST 1999

Does anybody has experience with allele-specific PCR. I´d like to have
some hints how the primers and conditions should be chosen before I start
to find out  these things again. To my knowledge only A-G C-G and A-A
mismaches will give results. 

What else can I do to make the reaction work?

Thanks a lot!


More information about the Methods mailing list