In Article <7c45t5$rt2$1 at news.doit.wisc.edu>,
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) wrote:
>1. Wash Sepharose CL-4B (not cross-linked version DOES NOT
>work!) with 10-20 volumes of pure water on glass filter.
I checked with Dima, and he clarified something for me. He means to say
that you must use the CL-4B, which is cross-linked, because plain 4B, which
is not cross linked, will not work with this activation method.
Department of Biological Sciences
University of Alberta
Edmonton, Alberta T6G 2E9
wgallin at gpu.srv.ualberta.ca