IUBio

DNA isolation from agarose gels

D. KIM dkim at NMSU.Edu
Tue Mar 9 16:38:30 EST 1999


A different way to make the "spin columns" described by Dr. Roychowdhury
is to place the gel slice on top of the aerosol-barrier of a cheap barrier
tip.  The more expensive barrier tips have a hydrophobic locking material
in the filter, making them useless for this application.

The tip and gel are then frozen, placed in a microtube and centrifuged as
described before.

Daniel Kim
(even cheaper than Hiryana!)



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