FIGE separation of YACs / yeast chromoosomes

David Johnston daj at nhm.ac.uk
Tue Mar 9 03:44:18 EST 1999


Hi,
we are currently trying to use FIGE to isolate YACs of mean size 300 KB
(range 100 KB to 1 MB) , in order to subclone. We are having real problems
identifying conditions that give reproducible results, seperate bands etc.

Please could anyone recommend:

% and type of agarose
buffer
run temp
sample loading techniques (plug vs liquid etc)
switching conditions

that give reliable results in the 100Kb to 1 MB range.

Thanks in advance,

David.

David A. Johnston,
Secretary to the WHO Schistosoma Genome Network,
Biomedical Parasitology Division,
Dept. of Zoology,
The Natural History Museum,
Cromwell Road, London SW7 5BD, England, UK.
Tel: 0171-938-9297 (from outside the UK: 44-171-938-9297)
Fax: 0171-938-9297 / 9249 / 8754 (from outside the UK: 44-171-938****)
eMail daj at nhm.ac.uk

http://www.nhm.ac.uk/hosted_sites/schisto/

The  Biomedical Parasitology Division is a WHO Collaborating Centre for the
identification of schistosomes and their snail hosts.





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