Need help w/ SDS & extraction buffer

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Wed Mar 10 11:24:15 EST 1999

In article <36E68937.28C6 at dogwood.botany.uga.edu>, Mark Warner
<mwarner at dogwood.botany.uga.edu> wrote:

> To all, I'm trying out a new RNA extraction buffer that contains Tris
> (0.2M), sodium perchlorate (8M)(pH 8.3) and SDS (2%), but the SDS comes
> out of solution and I can't seem to resolubilize it. I know this can
> happen with other buffers, and usually mild heat will do the trick, but
> it doessn't work. Any ideas?
> Mark

Maybe switch to sarkosyl as that is more salt and pH tolerant
(pH 8 and 8 M sounds somewhat out of the range for SDS).
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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