IUBio

Need help w/ SDS & extraction buffer

Mark Warner mwarner at dogwood.botany.uga.edu
Wed Mar 10 10:01:12 EST 1999


To all, I'm trying out a new RNA extraction buffer that contains Tris
(0.2M), sodium perchlorate (8M)(pH 8.3) and SDS (2%), but the SDS comes
out of solution and I can't seem to resolubilize it. I know this can
happen with other buffers, and usually mild heat will do the trick, but
it doessn't work. Any ideas?

Mark



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