Problems due protein accumulation on a RP-18 column.
lewr at bibo.lb.tu-berlin.de
Thu Mar 11 04:49:31 EST 1999
For quantifying amino acids, I use a OctaDeacetylSulphate 2 column.
I´ve got protein accumulation due an inadequate precolumn.
For purging the column, i´ve got two protocols:
60% isopropanol, 40% 4M urea
60% isopropanol, 40% 4M acetic acid
Does anybody have any recommendations about other protocols or
deviations like thiourea?
More information about the Methods