Problems due protein accumulation on a RP-18 column.

Klaus Throm lewr at bibo.lb.tu-berlin.de
Thu Mar 11 04:49:31 EST 1999


For quantifying amino acids, I use a OctaDeacetylSulphate 2 column.
I´ve got protein accumulation due an inadequate precolumn.
For purging the column, i´ve got two protocols:


     60% isopropanol, 40% 4M urea
     60% isopropanol, 40% 4M acetic acid


Does anybody have any recommendations about other protocols or
deviations like thiourea?

Thank you.




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