IUBio

Detection of GnHCl

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Thu Mar 11 13:54:37 EST 1999


:Richard, 
:
:You still remember the 1st year anorganic course?: If GuHCl is the 
:only one  source of chloride (halogen) ions in your solution, you 
:might check for this ion using silver nitrate solution. You'll get a 
:characteristic white precipitate that does not dissolve in diluted 
:nitric acid. Of course you also could measure chloride with a routine 
:analyzer in clinical chemistry, if you have access to any.
:

Yes, this is entirely valid approach. The amount of precipitate
can be quantified on 96 well microplate reader and converted to 
mM Cl- using calibrating curve. 

:> simple /chemical/ assay for guanidine hydrochloride? (we can't look
:> at conductivity).

Just curious - why? 

        - Dima



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