IUBio

strange effect in digesting cDNA library

Burkhard Hassel b.hassel at dkfz-heidelberg.de
Thu Mar 11 10:50:46 EST 1999


Hi netters!!

When I treated a cDNA (library) with XhoI, the sesult on the gel looked,
as if the cDNA smear shifted to a HIGHER molecular wheight.
cDNA 1st strand synth was performed using methylated dCTP, so XhoI (MBI,
Fermentas) should cut only in the site provided with the
oligo-dT-primer. The aim is an unidirectional cDNA library. Genomic DNA
was not visible in the poly-A RNA used.
Has anyone of you an idea?


Burkhard, Heidelberg




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