Fingerprinting of Balb/c mice

"Pouroullis Noula ", RD, RDD-R POUROULLIN at rdd.aeci.co.za
Fri Mar 12 00:57:03 EST 1999


>Is there anyone who could help me with any of the following questions,
>as I'm new to all types of eukaryotic work:
>I need to check the integrity of Balb/c mice i.e. check for
>contaminants, preferably using a molecular approach .
>
>1) what method is commonly used to extract DNA from mice?
>2) is there a std. set of markers that are commonly used to fingerprint
>Blab/c mice?
>3) how long does it take to set up and fingerprint using micro- or mini-
>satellites using manual methods.
>4) Would DIG-blotting or silver staining of mini/micro-satellite gels
>produce satisfactory fingerprints for analysis?
>Any suggestions will be appreciated
>Myrsini
>



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