Disposable spectrophotmetric cuvettes

Achim Recktenwald achimr at home.com
Thu Mar 11 21:43:19 EST 1999

Buy a flow-through cell, you get them as low as 5 - 10µL; if you do not have
a sipper for your spec, or a small pump you could use, do it with a syringe.


jdwalls at my-dejanews.com wrote in message
<7c9s78$6re$1 at nnrp1.dejanews.com>...
>In answer to your question, its for a colorimetric assay for biotin
>(HABA/Avidin). I'm quantitating biotin-label incorporation in in-vitro
>transcription reactions.  You measure the reduction in absorbance at 500nm
>when a biotin-containing sample is added to the HABA/Avidin solution.  When
>you're not reagent-limited, large volume cuvettes work fine for it.  I
>want to lose most of my IVT to a labeling efficiency assay though!
>Disposable cuvettes are preferable because of the waste-stream generated by
>this testing.  In order to rinse out the quartz cuvettes, you need to
>a lot of water with HABA (which is apparently quite toxic).  In addition,
>every time you handle a quartz cuvette you run the risk of breaking the
>things:  "Oops...tinkle...there goes another 250 bucks!" The Fisher ones
>the smallest I could find too, and they say the min volume is 1.2mL...

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net