Disposable spectrophotmetric cuvettes
achimr at home.com
Thu Mar 11 21:43:19 EST 1999
Buy a flow-through cell, you get them as low as 5 - 10µL; if you do not have
a sipper for your spec, or a small pump you could use, do it with a syringe.
jdwalls at my-dejanews.com wrote in message
<7c9s78$6re$1 at nnrp1.dejanews.com>...
>In answer to your question, its for a colorimetric assay for biotin
>(HABA/Avidin). I'm quantitating biotin-label incorporation in in-vitro
>transcription reactions. You measure the reduction in absorbance at 500nm
>when a biotin-containing sample is added to the HABA/Avidin solution. When
>you're not reagent-limited, large volume cuvettes work fine for it. I
>want to lose most of my IVT to a labeling efficiency assay though!
>Disposable cuvettes are preferable because of the waste-stream generated by
>this testing. In order to rinse out the quartz cuvettes, you need to
>a lot of water with HABA (which is apparently quite toxic). In addition,
>every time you handle a quartz cuvette you run the risk of breaking the
>things: "Oops...tinkle...there goes another 250 bucks!" The Fisher ones
>the smallest I could find too, and they say the min volume is 1.2mL...
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