>> If you give me more specifics like who made your column, what the pressure
> and the sive of the particles ,I might be able to give you more specific
Fist of all, thank you very much for your very fast and explicit help. I am
afraid but i think my description is incomplete.
The column is from the company KNAUER (Germany), 4.6*300[mm], material
SPHERIMAGE-80 Angstroem, particle size 5[µm], ODS-2(C18).