IUBio

Flag vs. myc epitope tags.

Svend Kjaer Svend at CAJAL.MBB.KI.SE
Fri Mar 12 03:51:19 EST 1999


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Dear all,

This is a recurrent thread. However, I have the choice to tag my scFv antibody
fragments with either of the two tags, so which one do you recommend and
preferably does anyone know of Kd determinations of the M2 and 9E10 antibody
for their respective tags, which would eliminate the "works fine in Western
blot at 1:10000 for me !" kind of responses.

best wishes,


Svend
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