Resue the Old Stock

brett at BORCIM.WUSTL.EDU brett at BORCIM.WUSTL.EDU
Fri Mar 12 08:55:37 EST 1999


>Dear Netters:
>Does any one has good protocol to save the old E.coli stock which
>maintained in -80 freezer for several years and cannot grow (which
>containing the plasmid I want)? Please reply to my Email as
>mqh at mh.bj.col.com.cn
>Thanks
>Dr Q. H. Ma
>Institute of Botany, Academia Sinica
>Beijing 100093, China


I have had this happen to me.  You can take the frozen stoock and miniprep it.
The plasmid yield will be low, but you can retransform it.





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