IUBio

Flag vs. myc epitope tags.

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Mar 12 07:44:13 EST 1999


Svend Kjaer <Svend at CAJAL.MBB.KI.SE> wrote:
> This is a recurrent thread. However, I have the choice to tag my scFv antibody
> fragments with either of the two tags, so which one do you recommend and
> preferably does anyone know of Kd determinations of the M2 and 9E10 antibody
> for their respective tags, which would eliminate the "works fine in Western
> blot at 1:10000 for me !" kind of responses.

Maybe it helps you to know that internal flag tags don't work. The tag has
to be either at the N or the C terminus.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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