IUBio

blunting: T4 polymerase or mung?

Burkhard Hassel b.hassel at dkfz-heidelberg.de
Fri Mar 12 04:30:53 EST 1999


Hi netters!

In the Clontech protocol to produce a 2hybrid cDNA library, the DNA is
blunted after the 2nd strand synthesis by simply adding some units of T4
DNA polymerase.
Wouldn't it be much more fail safe to use mung bean nuclease instead in
regard of hairpins?

Burkhard




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