Ethidium bromide decontamination

George Rutherford gruther at bilbo.bio.purdue.edu
Fri Mar 12 12:24:32 EST 1999


In article <lederer-1203991808110001 at iau60a.ak-i.med.uni-muenchen.de>,
lederer at pk-i.med.uni-muenchen.de (Erich) wrote:

> How do do decontaminate ethidium bromide bound in gels ?
> 
> I use do document my PCR  results by staining the agarose gels in an
> Etbr-bath.I decontaminate the staining solution by filtering it through
> carcoal and dispose the carcoal in a hazardous waste - bag which then goes
> thermal incineration (<262 °C). 
> But how do do treat your gels, gloves and cleaning tissues which may
> contain traces of Etbr ?
> Any suggestions appreciated - thanks

Although I hate to say it, the only practical and environmentally correct
thing to do is to toss all of these items in the hazardous waste. I
realize that this is expensive and not all that environmentally, but it's
about the only answer. 

George



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