Ari (apl at mail.student.oulu.fi) wrote:
: Is anybody really doing succesful primer sequencing with a pipeting robot?
: The total volumes used are small (7-10 micros), and some robots may not be
: accurate enough to handle it. Some or all of them?
: Do you have any good protocols for ABI 877 or maybe good experiences with
: other robots? If this robot won't work, we have to look for something
I have our Biomek 2000 doing BigDyePrimer reactions in 96 well plates
(cycled on MJ cyclers) and 384 well plates (cycled in PE dual 384 block
Viper). It takes about 30-40 minutes to setup 4 96-well DNA plates. They
are 5 or 6ul 1/4 BDP reactions done on M13. I have been very pleased
with it. I don't have the protocols on hand but you can e-mail me for them.
Paul Shinn ,___o
pshinn at neomorph.bio.upenn.edu _-\_<,
Arabidopsis thaliana Genome Center (*)/'(*)