Resue the Old Stock

Ricardo Silva ricardo.silva at CIGB.EDU.CU
Fri Mar 12 18:18:40 EST 1999


> From:           	mqh at BJ.COL.COM.CN (ma)
> Does any one has good protocol to save the old E.coli stock which
> maintained in -80 freezer for several years and cannot grow (which
> containing the plasmid I want)? Please reply to my Email as
> mqh at mh.bj.col.com.cn
> Thanks
> Dr Q. H. Ma
Take an aliquot, boil it, and use it directly for transformation.

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