> From: mqh at BJ.COL.COM.CN (ma)
> Does any one has good protocol to save the old E.coli stock which
> maintained in -80 freezer for several years and cannot grow (which
> containing the plasmid I want)? Please reply to my Email as
>mqh at mh.bj.col.com.cn> Thanks
> Dr Q. H. Ma
Take an aliquot, boil it, and use it directly for transformation.