George Rutherford wrote:
> In article <lederer-1203991808110001 at iau60a.ak-i.med.uni-muenchen.de>,
>lederer at pk-i.med.uni-muenchen.de (Erich) wrote:
>> > How do do decontaminate ethidium bromide bound in gels ?
> > I use do document my PCR results by staining the agarose gels in an
> > Etbr-bath.I decontaminate the staining solution by filtering it through
> > carcoal and dispose the carcoal in a hazardous waste - bag which then goes
> > thermal incineration (<262 °C).
> > But how do do treat your gels, gloves and cleaning tissues which may
> > contain traces of Etbr ?
> > Any suggestions appreciated - thanks
>> Although I hate to say it, the only practical and environmentally correct
> thing to do is to toss all of these items in the hazardous waste. I
> realize that this is expensive and not all that environmentally, but it's
> about the only answer.
I recall an old thread about EtBr and how to get rid of it. One person inquired
of the Municipal authorities in I believe Glasgow or Edinburgh in charge of
sewage, and they (as I recall) said rinse it down the drain. Another person
mentioned that EtBr is used during sheepshearing to disinfect any cuts the
animal gets with a brush loaded with EtBr. Check your wool clothing for EtBr
(This is meant as a joke).
Upshot (personal opinion): Dispose of properly, but don't go bananas.