PIPES solution (How do you get the PIPES to disolve?)

Graham Dellaire, Ph.D. Graham.Dellaire at hgu.mrc.ac.uk
Sat Mar 13 08:38:36 EST 1999


I am trying to make a 100 mM solution of PIPES and for the life of me I
can't get it into solution. Do you have to add acid or base.  I want to
have a final solution at a pH of 6.8.  I tried heating it to 50 degrees
etc... It just won't go into solution.

Anyone make up a solution with this stuff?

All and any help appreciated.... I will call Sigma on Monday in anycase.



Graham Dellaire

MRC Human Genetics Unit
Western General Hospital
Crewe Road
Edinburgh, UK

Fax: +44 (0)131 343 2620   
Phone: +44 (0)131 332 2471

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