IUBio

PIPES solution (How do you get the PIPES to disolve?)

Matthew Parker mparker at cmc.uab.edu
Sun Mar 14 18:15:20 EST 1999


Graham.Dellaire at hgu.mrc.ac.uk ("Graham Dellaire, Ph.D.") wrote:

>Heya,
>
>I am trying to make a 100 mM solution of PIPES and for the life of me I
>can't get it into solution. Do you have to add acid or base.  I want to
>have a final solution at a pH of 6.8.  I tried heating it to 50 degrees
>etc... It just won't go into solution.
>
>Anyone make up a solution with this stuff?
>
>
>-- 

Graham,

	The free acid is very difficult to dissolve unless the pH is
high beforehand. Use the salt instead.

	Matt



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