IUBio

PIPES solution (How do you get the PIPES to disolve?)

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Sun Mar 14 15:33:11 EST 1999


"Graham Dellaire, Ph.D." <Graham.Dellaire at hgu.mrc.ac.uk> wrote:
> I am trying to make a 100 mM solution of PIPES and for the life of me I
> can't get it into solution. Do you have to add acid or base.  I want to
> have a final solution at a pH of 6.8.

Depends on whether your pH now is above or below 6.8 (i.e. whether you use
a salt or the free acid).

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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