PIPES solution (How do you get the PIPES to disolve?)

Nicolas Lemee Lemee at ibm.net
Sun Mar 14 13:51:13 EST 1999


I think I used to use 1M KOH to disolve that stuff, takes a long time at RT,
but it does.
That was some time ago, I may be wrong...

--
Nicolas

"Graham Dellaire, Ph.D." wrote:

> Heya,
>
> I am trying to make a 100 mM solution of PIPES and for the life of me I
> can't get it into solution. Do you have to add acid or base.  I want to
> have a final solution at a pH of 6.8.  I tried heating it to 50 degrees
> etc... It just won't go into solution.
>
> Anyone make up a solution with this stuff?
>
> All and any help appreciated.... I will call Sigma on Monday in anycase.
>
> Cheers,
>
> Graham
>
> --
> ********************
> Graham Dellaire
>
> MRC Human Genetics Unit
> Western General Hospital
> Crewe Road
> Edinburgh, UK
> EH4 2XU
>
> Fax: +44 (0)131 343 2620
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