IUBio

Request: Vent PCR: advice on optimizing Longish PCR

S. Findley polliwog at u.washington.edu
Mon Mar 15 16:02:15 EST 1999


I am trying to amplify fragments of 2-3 kB with NEB's Vent polymerase.
I have heard that this enzyme doesn't like to please researchers trying to
amplify longer fragments.

What conditions have proven successful in optimizing/extending Vent
performance?

(Email responses preferred)

Thanks,

SDF
-- 
Seth D. Findley               polliwog at u.washington.edu
Department of Biochemistry    Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195



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