I am trying to amplify fragments of 2-3 kB with NEB's Vent polymerase.
I have heard that this enzyme doesn't like to please researchers trying to
amplify longer fragments.
What conditions have proven successful in optimizing/extending Vent
(Email responses preferred)
Seth D. Findley polliwog at u.washington.edu
Department of Biochemistry Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195