G418 is used for eukaryotic cells, neomycin for bacterial cells. The way I
understand it, the resistance factor is the same but you need the appropriate
promoter to express it depending on the organism used.
In article <36E88E23.9FA83B49 at rcyllene.uwa.edu.au>, mwatson at rcyllene.uwa.edu.au
>>A question from a non microbiologist. If a plasmid has neomycin
>selection why can't you use neomycin as the selection antibiotic?
>Everyone uses G418 (Geneticin). What is the basis for using
>G418 vs neo? Toxicity? Cost? some other reason that I'm just too
>dumb to figure out :-)
>Dr Mark Watson B.Sc.(Hon) Ph.D.
>Details at http://www.cbsm.uwa.edu.au/sigfile.html>>